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Literature summary for 2.5.1.135 extracted from

  • Zheng, L.; Zhou, X.; Zhang, H.; Ji, X.; Li, L.; Huang, L.; Bai, L.; Zhang, H.
    Structural and functional analysis of validoxylamine A 7-phosphate synthase ValL involved in validamycin A biosynthesis (2012), PLoS ONE, 7, e32033.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.135

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces hygroscopicus subsp. jinggangensis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol 4000, 200 mM MgCl2, 100 mM Tris-HCl (pH 8.5) Streptomyces hygroscopicus subsp. jinggangensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-valienol + validamine 7-phosphate Streptomyces hygroscopicus subsp. jinggangensis
-
 validoxylamine A 7'-phosphate + GDP
-
 ?
GDP-valienol + validamine 7-phosphate Streptomyces hygroscopicus subsp. jinggangensis 5008
-
 validoxylamine A 7'-phosphate + GDP
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces hygroscopicus subsp. jinggangensis Q1L2L2
-
-
Streptomyces hygroscopicus subsp. jinggangensis 5008 Q1L2L2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography and Superdex 75 gel filtration Streptomyces hygroscopicus subsp. jinggangensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-valienol + validamine 7-phosphate
-
 Streptomyces hygroscopicus subsp. jinggangensis validoxylamine A 7'-phosphate + GDP
-
 ?
GDP-valienol + validamine 7-phosphate
-
 Streptomyces hygroscopicus subsp. jinggangensis 5008 validoxylamine A 7'-phosphate + GDP
-
 ?

Synonyms

Synonyms Comment Organism
V7P synthase
-
 Streptomyces hygroscopicus subsp. jinggangensis
validoxylamine A 7-phosphate synthase
-
 Streptomyces hygroscopicus subsp. jinggangensis
valL
-
 Streptomyces hygroscopicus subsp. jinggangensis

General Information

General Information Comment Organism
malfunction enzyme inactivation abolishes validamycin A production Streptomyces hygroscopicus subsp. jinggangensis
metabolism the enzyme is an essential part of the validamycin biosynthesis pathway Streptomyces hygroscopicus subsp. jinggangensis